TRANSGLYCOSYLATION AND MULTIPLE ATTACK OF ENDO-(1-]3)-BETA-D-GLUCANASE-L-IV FROM SPISULA-SACHALINENSIS - A NEW APPROACH TO THE EVALUATION OF THE DEGREE OF MULTIPLE ATTACK ON POLYSACCHARIDES

The marked capability for transglycosylation by the endo-(1→3)-β-d-glucanase L-IV from Spisula sachalinensis to glycerol, d-glucose, methyl a- and β-d-glucopyranoside, and methyl cellobioside as acceptors was employed to study the multiple attack of the enzyme on laminarin. The enzyme hydrolyses, on average, ∼4 glycosidic bonds during enzyme-substrate interaction. The relative transfer constants for each acceptor have been calculated. © 1990.