PURIFICATION OF THE CENTROMERE-SPECIFIC PROTEIN CENP-A AND DEMONSTRATION THAT IT IS A DISTINCTIVE HISTONE

被引：320

作者：

PALMER, DK ^{[1
]}

ODAY, K ^{[1
]}

TRONG, HL ^{[1
]}

CHARBONNEAU, H ^{[1
]}

MARGOLIS, RL ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT BIOCHEM SJ70,SEATTLE,WA 98195

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 09期

关键词：

HISTONE H3; SCLERODERMA; AUTOANTIBODIES; KINETOCHORE; CHROMATIN;

D O I：

10.1073/pnas.88.9.3734

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

CENP-A, a centromere-specific 17-kDa protein, has histone-like properties. However, in contrast to the common somatic histones, CENP-A is quantitatively retained in bull spermatozoa, and we have exploited this fact to purify CENP-A to apparent homogeneity. Partial sequence analysis of the purified protein indicates that CENP-A is a distinctive gene product. Some CENP-A sequences are highly similar to regions of histone H3. Other segments of CENP-A are not related to H3 or any other histone. These unrelated segments are presumably involved in localizing CENP-A to centromeric DNA or in centromere-specific functions of CENP-A.

引用

页码：3734 / 3738

页数：5

共 30 条

[1] INJECTION OF ANTICENTROMERE ANTIBODIES IN INTERPHASE DISRUPTS EVENTS REQUIRED FOR CHROMOSOME MOVEMENT AT MITOSIS
BERNAT, RL
BORISY, GG
ROTHFIELD, NF
EARNSHAW, WC
[J]. JOURNAL OF CELL BIOLOGY, 1990, 111 (04) : 1519 - 1533
[2] A 47-KDA HUMAN NUCLEAR-PROTEIN RECOGNIZED BY ANTIKINETOCHORE AUTOIMMUNE SERA IS HOMOLOGOUS WITH THE PROTEIN ENCODED BY RCC1, A GENE IMPLICATED IN ONSET OF CHROMOSOME CONDENSATION
BISCHOFF, FR
MAIER, G
TILZ, G
PONSTINGL, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) : 8617 - 8621
[3] KINETOCHORE STRUCTURE, DUPLICATION, AND DISTRIBUTION IN MAMMALIAN-CELLS - ANALYSIS BY HUMAN AUTOANTIBODIES FROM SCLERODERMA PATIENTS
BRENNER, S
PEPPER, D
BERNS, MW
TAN, E
BRINKLEY, BR
[J]. JOURNAL OF CELL BIOLOGY, 1981, 91 (01) : 95 - 102
[4] COOKE CA, 1990, J CELL BIOL, V110, P1479
[5] DAYHOFF MO, 1983, METHOD ENZYMOL, V91, P524
[6] DELANGE RJ, 1973, J BIOL CHEM, V248, P3261
[7] THE KINETOCHORE IS PART OF THE METAPHASE CHROMOSOME SCAFFOLD
EARNSHAW, WC
HALLIGAN, N
COOKE, C
ROTHFIELD, N
[J]. JOURNAL OF CELL BIOLOGY, 1984, 98 (01) : 352 - 357
[8] IDENTIFICATION OF A FAMILY OF HUMAN CENTROMERE PROTEINS USING AUTOIMMUNE SERA FROM PATIENTS WITH SCLERODERMA
EARNSHAW, WC
ROTHFIELD, N
[J]. CHROMOSOMA, 1985, 91 (3-4) : 313 - 321
[9] MOLECULAR-CLONING OF CDNA FOR CENP-B, THE MAJOR HUMAN CENTROMERE AUTOANTIGEN
EARNSHAW, WC
SULLIVAN, KF
MACHLIN, PS
COOKE, CA
KAISER, DA
POLLARD, TD
ROTHFIELD, NF
CLEVELAND, DW
[J]. JOURNAL OF CELL BIOLOGY, 1987, 104 (04) : 817 - 829
[10] POSTTRANSLATIONAL GLUTAMYLATION OF ALPHA-TUBULIN
EDDE, B
ROSSIER, J
LECAER, JP
DESBRUYERES, E
GROS, F
DENOULET, P
[J]. SCIENCE, 1990, 247 (4938) : 83 - 85

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