CHARACTERIZATION OF LIVER-SPECIFIC EXPRESSION OF RAT URICASE USING MONOCLONAL-ANTIBODIES AND CLONED CDNAS

被引：21

作者：

MOTOJIMA, K

GOTO, S

机构：

[1] Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Funabashi, Chiba

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1087卷 / 03期

关键词：

(Hepatocyte); (Rat liver); Liver-specific gene expression; Monoclonal antibody; Peroxisome; Uricase;

D O I：

10.1016/0167-4781(90)90005-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tissue distribution of uricase (urate oxidase, EC 1.7.3.3) was studied by immunoblotting and RNA slot blot analysis. For immunoblotting, highly specific monoclonal antibodies against rat liver uricase were obtained, and for mRNA detection, a cloned uricase cDNA was used. Among seven tissues studied, uricase was immunologically detected only in the liver. The contents of uricase in other tissues, i.e., brain, thymus, heart, spleen, kidney and lactating mammary gland, were estimated to be less than 2% of that in the liver. Uricase mRNA was also detected only in the liver. The steady-state level of the mRNA in the isolated hepatocytes was relatively constant during the 8-day culture period when compared with those of other mRNAs expressed in the liver, suggesting a unique control mechanism of its expression. © 1990.

引用

收藏

页码：316 / 322

页数：7

相关论文

共 29 条

[1]

ANDERSON D N W, 1982, International Tree Crops Journal, V2, P15

[2] URATE OXIDASE IN PRIMATE PHYLOGENESIS [J].

CHRISTEN, P ;

PEACOCK, WC ;

CHRISTEN, AE ;

WACKER, WEC .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 12 (01) :3-&

[3] CHANGES IN LIVER-SPECIFIC COMPARED TO COMMON GENE-TRANSCRIPTION DURING PRIMARY CULTURE OF MOUSE HEPATOCYTES [J].

CLAYTON, DF ;

DARNELL, JE .

MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (09) :1552-1561

[4] HIGH PREALBUMIN AND TRANSFERRIN MESSENGER-RNA LEVELS IN THE CHOROID-PLEXUS OF RAT-BRAIN [J].

DICKSON, PW ;

ALDRED, AR ;

MARLEY, PD ;

TU, GF ;

HOWLETT, GJ ;

SCHREIBER, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 127 (03) :890-895

[5] APOLIPOPROTEIN-E MESSENGER-RNA IS ABUNDANT IN THE BRAIN AND ADRENALS, AS WELL AS IN THE LIVER, AND IS PRESENT IN OTHER PERIPHERAL-TISSUES OF RATS AND MARMOSETS [J].

ELSHOURBAGY, NA ;

LIAO, WS ;

MAHLEY, RW ;

TAYLOR, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (01) :203-207

[6] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].

FAIRBANKS, G ;

STECK, TL ;

WALLACH, DFH .

BIOCHEMISTRY, 1971, 10 (13) :2606-+

[7] COMPARISON OF INTRAPEROXISOMAL LOCALIZATION FORM AND PROPERTIES OF AMPHIBIAN (RANA-CATESBEIANA) URICASE WITH THOSE OF OTHER ANIMAL URICASES [J].

FUJIWARA, S ;

OHASHI, H ;

NOGUCHI, T .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1987, 86 (01) :23-26

[8] IDENTIFICATION OF PEROXISOMAL TARGETING SIGNALS LOCATED AT THE CARBOXY TERMINUS OF 4 PEROXISOMAL PROTEINS [J].

GOULD, SJ ;

KELLER, GA ;

SUBRAMANI, S .

JOURNAL OF CELL BIOLOGY, 1988, 107 (03) :897-905

[9] A TOTAL EXTRACT DOT BLOT HYBRIDIZATION PROCEDURE FOR MESSENGER-RNA QUANTITATION IN SMALL SAMPLES OF TISSUES OR CULTURED-CELLS [J].

GRIMES, A ;

MCARDLE, HJ ;

MERCER, JFB .

ANALYTICAL BIOCHEMISTRY, 1988, 172 (02) :436-443

[10] PERSISTENCE OF LIVER-SPECIFIC MESSENGER-RNA IN CULTURED-HEPATOCYTES - DIFFERENT REGULATORY EVENTS FOR DIFFERENT GENES [J].

ISOM, H ;

GEORGOFF, I ;

SALDITTGEORGIEFF, M ;

DARNELL, JE .

JOURNAL OF CELL BIOLOGY, 1987, 105 (06) :2877-2885