COACTIVATOR AND PROMOTER-SELECTIVE PROPERTIES OF RNA-POLYMERASE-I TAFS

被引：84

作者：

BECKMANN, H ^{[1
]}

CHEN, JL ^{[1
]}

OBRIEN, T ^{[1
]}

TJIAN, R ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA

来源：

SCIENCE | 1995年 / 270卷 / 5241期

关键词：

D O I：

10.1126/science.270.5241.1506

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human ribosomal RNA synthesis by RNA polymerase I requires the activator UBF and the promoter selectivity factor SL1, which consists of the TATA binding protein (TBP) and three associated subunits, TAF(I) 110, TAF(I)63, and TAF(I)48. Here it is shown that both TAF(I)110 and TAF(I)63 contact the promoter, whereas TAF(l)48 serves as a target for interaction with UBF and is required to form a transcriptionally active SL1 complex responsive to UBF in vitro. TAF(I)48 also alters the ability of TBP to interact with TATA box elements, and the resulting complex fails to support transcription by RNA polymerase II. Thus, TAF(I)48 may function both as a target to mediate UBF activation and as a class-specific promoter selectivity factor.

引用

页码：1506 / 1509

页数：4

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