TISSUE-SPECIFIC SORTING OF THE HUMAN LDL RECEPTOR IN POLARIZED EPITHELIA OF TRANSGENIC MICE

被引:98
作者
PATHAK, RK
YOKODE, M
HAMMER, RE
HOFMANN, SL
BROWN, MS
GOLDSTEIN, JL
ANDERSON, RGW
机构
[1] UNIV TEXAS,SW MED CTR,DEPT NEUROSCI,DALLAS,TX 75235
[2] UNIV TEXAS,SW MED CTR,DEPT MOLEC GENET,DALLAS,TX 75235
[3] UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST,DALLAS,TX 75235
关键词
D O I
10.1083/jcb.111.2.347
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The distribution of human low density lipoprotein (LDL) receptors was studied by immunofluorescence and immunoelectron microscopy in epithelial cells of transgenic mice that express high levels of receptors under control of the metallothionein-I promoter. In hepatocytes and intestinal epithelial cells, the receptors were confined to the basal and basolateral surfaces, respectively. Very few LDL receptors were present in coated pits or intracellular vesicles. In striking contrast, in the epithelium of the renal tubule the receptors were present on the apical (lumenal) surface where they appeared to be concentrated at the base of microvilli and were abundant in vesicles of the endocytic recycling pathway. Intravenously administered LDL colloidal gold conjugates bound to the receptors on hepatocyte microvilli and were slowly internalized, apparently through slow migration into coated pits. We conclude that (a) sorting of LDL receptors to the surface of different epithelial cells varies with each tissue; and (b) in addition to a signal for clustering in coated pits, the LDL receptor may contain a signal for retention in noncoated membrane that is manifest in hepatocytes and intestinal epithelial cells, but not in renal epithelial cells or cultured human fibroblasts.
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页码:347 / 359
页数:13
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