FATTY-ACID INCORPORATION INTO MEMBRANES OF DISPERSED RAT SUBMANDIBULAR SALIVARY-GLAND CELLS AND THEIR EFFECT ON ADENYLATE-CYCLASE ACTIVITY

被引：2

作者：

AHMAD, SN ^{[1
]}

ALAM, SQ ^{[1
]}

ALAM, BS ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,NEW ORLEANS,LA 70119

来源：

ARCHIVES OF ORAL BIOLOGY | 1990年 / 35卷 / 11期

关键词：

ADENYLATE CYCLASE; SALIVARY GLAND CELLS; MEMBRANE FATTY ACIDS;

D O I：

10.1016/0003-9969(90)90066-J

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Dispersed cells from rat submandibular salivary glands were incubated in medium supplemented with different fatty acids, such as stearic (18:0), oleic (cis 18:1 omega 9) and elaidic acid (trans 18:1 omega 9). The exogenous fatty acids were incorporated into the membrane lipids. A comparison of the adenylate cyclase activity in membranes of cells incubated with different fatty acids revealed the following order (from highest to lowest specific activity): steric, alaidic, oleic. The findings suggest that changes in adenylate cyclase activity in these dispersed membranes enriched with different fatty acids may be related to the possible changes in membrane fluidity. The synthesis of glycoproteins was studied by measuring the incorporation of [1-C-14]-glucosamine into TCA-PTA-precipitable material in the presence or absence of fatty acids. No effect of the exogenous fatty acids on glycoprotein synthesis was observed.

引用

页码：879 / 883

页数：5

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