REASSESSMENT OF PLASMA ANGIOTENSINS MEASUREMENT - EFFECTS OF PROTEASE INHIBITORS AND SAMPLE HANDLING PROCEDURES

被引：84

作者：

KOHARA, K ^{[1
]}

TABUCHI, Y ^{[1
]}

SENANAYAKE, P ^{[1
]}

BROSNIHAN, KB ^{[1
]}

FERRARIO, CM ^{[1
]}

机构：

[1] CLEVELAND CLIN FDN, RES INST,DEPT BRAIN & VASC RES,9500 EUCLID AVE, 1 CLIN CTR, CLEVELAND, OH 44195 USA

来源：

PEPTIDES | 1991年 / 12卷 / 05期

关键词：

ANGIOTENSIN-I; ANGIOTENSIN-II; ANGIOTENSIN(1-7); ANGIOTENSINASES; PROTEASE INHIBITORS; HPLC; RADIOIMMUNOASSAYS; AMINOPEPTIDASES; CONVERTING ENZYME; PROLYL ENDOPEPTIDASE;

D O I：

10.1016/0196-9781(91)90070-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Characterization of C- and N-terminal forms of angiotensin (Ang) peptides mandated assessment of methods to determine plasma levels. I-125-Ang I, I-125-Ang II, and I-125-Ang(1-7) were added to blood samples in the presence of protease inhibitors. Ethylenediaminetetraacetic acid (EDTA) inhibited the conversion of I-125-Ang I to I-125-Ang II. o-Phenanthroline and EDTA (EDTA + o-Ph) did not eliminate [des-Asp1) fragments or I-125-Ang(1-7). The combination of EDTA + o-Ph and pepstatin A or 4-(chloromercuri) benzoic acid (PCMB) significantly reduced I-125-Ang(1-7) generation. Only PCMB plus EDTA + o-Ph eliminated [des-Asp1] fragments. Authentic plasma values of Ang peptides require the correct choice of protease inhibitors.

引用

页码：1135 / 1141

页数：7

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