IDENTIFICATION OF THE PROMOTER REGION OF THE ESCHERICHIA-COLI MAJOR COLD SHOCK GENE, CSPA

被引：104

作者：

TANABE, H ^{[1
]}

GOLDSTEIN, J ^{[1
]}

YANG, MZ ^{[1
]}

INOUYE, M ^{[1
]}

机构：

[1] UNIV MED & DENT NEW JERSEY,RUTGERS MED SCH,ROBERT WOOD JOHNSON MED SCH,DEPT BIOCHEM,PISCATAWAY,NJ 08854

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 12期

关键词：

D O I：

10.1128/jb.174.12.3867-3873.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The major cold shock protein of Escherichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10-degrees-C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstable at 37-degrees-C, so that CS7.4 production was hardly detected when the culture temperature was shifted from 15-degrees-C to 37-degrees-C. The transcription initiation site (+1) was identified. In vivo footprinting demonstrated that the region from bases -35 to -73 was protected from chemical modification, and gel mobility shift analysis showed that a cold-shocked cell extract contained a factor(s) specifically bound to the fragment containing the sequence between bases -63 and -92. This factor was synthesized de novo only at low temperature, and its synthesis was inhibited by chloramphenicol. Possible functions of this factor are discussed.

引用

页码：3867 / 3873

页数：7

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