学术探索
学术期刊
新闻热点
数据分析
智能评审

CENTRIOLE DUPLICATION IN LYSATES OF SPISULA-SOLIDISSIMA OOCYTES

被引：88
作者：
PALAZZO, RE
VAISBERG, E
COLE, RW
RIEDER, CL
机构：
[1] ACAD SCI USSR,PROT RES INST,MOSCOW V-71,USSR
[2] WADSWORTH CTR LABS & RES,ALBANY,NY 12201
[3] SUNY ALBANY,DEPT BIOMED SCI,ALBANY,NY 12222
来源：
SCIENCE | 1992年 / 256卷 / 5054期
关键词：
D O I：
10.1126/science.1566068
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A cell-free system has been developed that executes centriole duplication. Surf clam (Spisula solidissima) oocytes, arrested at late prophase of meiosis I, do not contain centrioles, centrosomes, or asters. Serial section high-voltage electron microscopy (HVEM) of asters and spindles isolated from potassium chloride-activated oocytes indicates that within 4 minutes oocytes assemble a single centriole that is duplicated by 15 minutes when assembly of the first meiotic spindle is complete. A mixture of lysates from unactivated oocytes and potassium chloride-activated oocytes induces centriole formation and duplication. Astral microtubule content in these lysate mixtures increases with time.
引用
收藏
页码：219 / 221
页数：3
相关论文
共 22 条
123