THE DEVELOPMENTAL COMPETENCE OF BOVINE EMBRYOS WITH REDUCED CELL NUMBERS

被引:44
作者
LOSKUTOFF, NM [1 ]
JOHNSON, WH [1 ]
BETTERIDGE, KJ [1 ]
机构
[1] UNIV GUELPH, ONTARIO VET COLL, DEPT POPULAT MED, GUELPH N1G 2W1, ONTARIO, CANADA
基金
加拿大自然科学与工程研究理事会;
关键词
CATTLE; MICROMANIPULATION; SPLITTING; BLASTOMERE; INVITRO FERTILIZATION;
D O I
10.1016/0093-691X(93)90026-2
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Early cleavage stage bovine embryos can now be readily produced and maintained in vitro; therefore, their usefulness for producing identical calves and providing biopsies for sexing was investigated and compared with a conventional method of embryo splitting. Up to half the total number of blastomeres can be removed from 2- to 16-cell embryos without adversely affecting their subsequent development into blastocysts. Two- and 4-cell embryos needed to be maintained at 38-degrees-C during manipulation because of their sensitivity to cooling. Until the time of hatching, cell numbers in such blastocysts were reduced in proportion to the numbers of cells originally removed. The transfer of two demi-embryos produced from bisected blastocysts resulted in pregnancy rates similar to that obtained with whole embryos and there were no differences between cell numbers in, or survival of, demi-embryos produced in vitro or in vivo. Multiple calves also have been produced from single blastomeres isolated from 4-cell embryos produced in vitro. Seventy three percent of the 4-cell embryos manipulated produced either 3 or 4 blastocysts. Pregnancy rates resulting from transferred pairs of quarter embryos were lower than from demi-embryos, but there are some indications that success rates may be increased if the smaller embryos are co-transferred with fresh trophoblastic vesicles.
引用
收藏
页码:95 / 107
页数:13
相关论文
共 58 条
  • [1] DEVELOPMENT OF DAY-7 BOVINE DEMI-EMBRYOS TRANSFERRED INTO VIRGIN AND REPEAT-BREEDER HEIFERS
    ALBIHN, A
    GUSTAFSSON, H
    RODRIGUEZMARTINEZ, H
    LARSSON, K
    [J]. ANIMAL REPRODUCTION SCIENCE, 1989, 21 (3-4) : 161 - 176
  • [2] ALLEN WR, 1984, J REPROD FERTIL, V71, P607, DOI 10.1530/jrf.0.0710607
  • [3] Betteridge K. J., 1989, Evolutionary mechanisms in sex determination., P279
  • [4] THE USE OF MALE-SPECIFIC CHROMOSOMAL DNA FRAGMENTS TO DETERMINE THE SEX OF BOVINE PREIMPLANTATION EMBRYOS
    BONDIOLI, KR
    ELLIS, SB
    PRYOR, JH
    WILLIAMS, MW
    HARPOLD, MM
    [J]. THERIOGENOLOGY, 1989, 31 (01) : 95 - 104
  • [5] FREEZING CATTLE DEMI-EMBYOS - INFLUENCE OF A PERIOD OF CULTURE BETWEEN SPLITTING AND FREEZING ON SURVIVAL
    CHESNE, P
    HEYMAN, Y
    CHUPIN, D
    PROCUREUR, R
    MENEZO, Y
    [J]. THERIOGENOLOGY, 1987, 27 (01) : 218 - 218
  • [6] FEHILLY CB, 1986, OXFORD REV REPROD B, V8, P379
  • [7] FREEMAN AE, 1992, P S CLONING MAMMALS, P17
  • [8] FUKUI Y, 1989, J REPROD FERTIL, V86, P501, DOI 10.1530/jrf.0.0860501
  • [9] THE COMMERCIAL APPLICATION OF EMBRYO SPLITTING IN BEEF-CATTLE
    GRAY, KR
    BONDIOLI, KR
    BETTS, CL
    [J]. THERIOGENOLOGY, 1991, 35 (01) : 37 - 43
  • [10] INVIVO DEVELOPMENT OF INVITRO FERTILIZED BOVINE OOCYTES MATURED INVIVO VERSUS INVITRO
    GREVE, T
    XU, KP
    CALLESEN, H
    HYTTEL, P
    [J]. JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER, 1987, 4 (05): : 281 - 285