1 KB OF THE LACTASE-PHLORHIZIN HYDROLASE PROMOTER DIRECTS POST-WEANING DECLINE AND SMALL INTESTINAL-SPECIFIC EXPRESSION IN TRANSGENIC MICE

被引：49

作者：

TROELSEN, JT

MEHLUM, A

OLSEN, J

SPODSBERG, N

HANSEN, GH

PRYDZ, H

NOREN, O

SJOSTROM, H

机构：

[1] UNIV OSLO, UNIV CLIN, DEPT ANIM, N-0316 OSLO 3, NORWAY

[2] BIOTECHNOL CTR OSLO, N-0316 OSLO 3, NORWAY

来源：

FEBS LETTERS | 1994年 / 342卷 / 03期

关键词：

LACTASE-PHLORHIZIN HYDROLASE; TISSUE SPECIFICITY; SMALL INTESTINE; ENTEROCYTE; HYPOLACTASIA; LACTOSE INTOLERANCE;

D O I：

10.1016/0014-5793(94)80519-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.

引用

页码：291 / 296

页数：6

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