Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex

被引：16

作者：

Hiort, C ^{[1
]}

Goodisman, J ^{[1
]}

Dabrowiak, JC ^{[1
]}

机构：

[1] SYRACUSE UNIV,DEPT CHEM,SYRACUSE,NY 13244

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1995年 / 153卷 / 1-2期

关键词：

DNA cleavage; insulin-mimetic; bisperoxovanadium; photolysis; photocleavage;

D O I：

10.1007/BF01075916

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH4[VQ(O-2)(2)-(1,10-phenanthroline)], bpV(phen), have been studied. V-51 NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.

引用

收藏

页码：31 / 36

页数：6

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