HIGHLY SENSITIVE DETERMINATION OF PHOTOSYNTHETIC PIGMENTS IN MARINE INSITU SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：32

作者：

KOHATA, K ^{[1
]}

WATANABE, M ^{[1
]}

YAMANAKA, K ^{[1
]}

机构：

[1] SCI UNIV TOKYO,DEPT IND CHEM,SHINJUKU KU,TOKYO 162,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 558卷 / 01期

关键词：

D O I：

10.1016/0021-9673(91)80118-Z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic (HPLC) method was developed by optimizing a solvent system for the highly sensitive determination of photosynthetic pigments in marine in situ samples. The proposed HPLC system can handle injection volumes greater than 400-mu-l without a significant loss in the resolution between chlorophyllide a and chlorophyll c1 + c2. The system also reduces the sample volumes required for the analysis of typical pigments of oligotrophic sea water to 500 ml. The elution time for all pigments is 24 min at a flow-rate of 1 ml/min and 12 min at a flow-rate of 2 ml/min using a 250 mm x 4.6 mm I.D. column.

引用

页码：131 / 140

页数：10

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