HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF SULFONATED AROMATICS USING A BETA-CYCLODEXTRIN-BONDED PHASE

A cyclodextrin-bonded phase and methanol-aqueous ammonium acetate mobile phase were used in the liquid chromatographic analysis of a number of sulfonated aromatics. This separation system offered two advantages over the more conventional ion-pair approach to the liquid chromatographic analysis of these highly polar compounds. It achieved the resolution of positional isomers of sulfonated aromatics and allowed the use of thermospray liquid chromatography/mass spectrometry for the characterization of these compounds. Chromatographic selectivity for these solutes was found to be most significantly influenced by the ionic strength of the mobile phase mixture.