BASIC FIBROBLAST GROWTH-FACTOR SELECTIVELY AMPLIFIES THE FUNCTIONAL-STATE OF NEURONS PRODUCING NEUROPEPTIDE-Y BUT NOT SOMATOSTATIN IN CULTURES OF FETAL BRAIN-CELLS - EVIDENCE FOR A COOPERATIVE INTERACTION WITH INSULIN-LIKE GROWTH FACTOR-I

The role of basic fibroblast growth factor (bFGF) in regulating the functional state of neuropeptide Y (NPY) neurons in the brain was investigated, using aggregate cultures, derived from 17-day-old fetal mt cortex maintained for 16 days in serum-free medium, as a model. The criterion for the functional state was NPY production in response to a 24-h exposure to forskolin+phorbol 12-myristate 13-acetate (For+PMA). bFGF (0.1 nM) induced a almost-equal-to 2-fold increase in NPY production under basal conditions as well as after For+PMA (p < 0.001 vs control). To address the possibility 'that bFGF way interact with other growth factors, we assessed the effect of bFGF in the presence of long R3-insulin-like growth factor-I (l-IGF-I; 1 nM) and found that NPY production in response to For+PMA was even greater than with bFGF alone (2-fold; p < 0.001); even though l-IGF-I by itself was ineffective; suggesting that bFGF is the driving force of this amplification. To assess the selectivity of this process, we evaluated SRIF production in response to For+PMA and found that it was not amplified by bFGF, l-IGF-I, or bFGF+l-IGF-I. These results are consistent with bFGF selectively amplifying the functional state of the cAMP and protein kinase C (PKC) pathways leading to increased NPY-production, with cooperative interaction(s) between bFGF and IGF-I, and with a role for bFGF and IGF-I in the developmental expression/survival of the NPY neurons.