DEFICIENT DNA-REPAIR OF TRIPLE HELIX-DIRECTED DOUBLE PSORALEN DAMAGE IN HUMAN-CELLS

被引:18
作者
SANDOR, Z
BREDBERG, A
机构
[1] Department of Medical Microbiology, University of Lund, General Hospital
关键词
TRIPLE HELIX DNA; PSORALEN; MUTAGENESIS; DNA REPAIR; SV40 SHUTTLE VECTOR;
D O I
10.1016/0014-5793(95)01133-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Damage induced by a single psoralen-modified triple helix-forming oligonucleotide has been reported to be efficiently repaired in human cells, In this study we investigated a set of psoralen coupled oligonucleotides introducing multiple lesions into the target DNA. A simian virus 40 (SV40) shuttle vector was in vitro treated with different triple helix-forming oligonucleotides and UVA radiation, leading to double psoralen adducts at the supF mutational target gene of the plasmid, After passage in the Rail human cell line the recovered vector was analysed in an indicator bacterial strain, The results show that double psoralen adducts, located at both ends of a long triple helix, cannot be repaired efficiently in human cells.
引用
收藏
页码:287 / 291
页数:5
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