ENZYMATIC PRODUCTION AND ISOLATION OF D-AMINO ACIDS FROM THE CORRESPONDING 5-SUBSTITUTED HYDANTOINS

被引：14

作者：

DEEPA, S

SIVASANKAR, B

JAYARAMAN, K

PRABHAKARAN, K

GEORGE, S

PALANI, P

RAMESH, KS

SRINIVASAN, CV

KANDASAMY, NR

SADHUKHAN, AK

机构：

[1] SO PETROCHEM IND CORP LTD, PHARMA, RES & DEV CTR, MARAIMALAI NAGAR 603209, TAMIL NADU, INDIA

[2] ANNA UNIV, CTR BIOTECHNOL, MADRAS 600025, INDIA

来源：

PROCESS BIOCHEMISTRY | 1993年 / 28卷 / 07期

关键词：

D O I：

10.1016/0032-9592(93)85027-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A detailed process for the production of D-amino acids starting from the synthesis of 5-substituted hydantoin was established using whole cells of Agrobacterium radiobacter. About 94% substrate turnover was attained at 30 mg/ml substrate concentration. On isolation and concentration of the end product, 65% recovery was obtained. The product was found to be 99% pure by high performance liquid chromatography (HPLC) and infrared (IR) analysis, and confirmed by optical rotation.

引用

页码：447 / 452

页数：6

共 11 条

[1] Identification of carbonyl compounds through conversion into hydantoins [J].