PREPARATION OF ISOLATED BIOMOLECULES FOR SFM OBSERVATIONS - BACTERIOPHAGE-T4 AS A TEST SAMPLE

被引：12

作者：

DROZ, E ^{[1
]}

TABORELLI, M ^{[1
]}

WELLS, TNC ^{[1
]}

DESCOUTS, P ^{[1
]}

机构：

[1] GLAXO INST MOLEC BIOL,GENEVA,SWITZERLAND

来源：

BIOPHYSICAL JOURNAL | 1993年 / 65卷 / 03期

关键词：

D O I：

10.1016/S0006-3495(93)81174-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The T4 bacteriophage has been used to investigate protocols for the preparation of samples for scanning force microscopy in air, in order to obtaining reproducible images. The resolution of images and the distribution of bacteriophages on the substrate depends on the buffer type, its concentration, the surface treatment of substrate, and the method of deposition. The best imaging conditions for the phages require dilution in a volatile buffer at low ionic strength and adsorption onto hydrophilic surfaces. When imaging with the scanning force microscopy the quality of the images is influenced by the vertical and lateral forces applied on the sample and by the tip geometry.

引用

页码：1180 / 1187

页数：8

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