RADIOIMMUNOASSAY OF 3-ALPHA-HYDROXY-5-ALPHA-PREGNAN-20-ONE IN RAT AND HUMAN PLASMA

被引：202

作者：

PURDY, RH

MOORE, PH

RAO, PN

HAGINO, N

YAMAGUCHI, T

SCHMIDT, P

RUBINOW, DR

MORROW, AL

PAUL, SM

机构：

[1] Department of Organic Chemistry, Southwest Foundation for Biomedical Research, San Antonio, TX

[2] Department of Cellular and Structural Biology, The University of Texas Health Science Center, San Antonio, TX

[3] Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, MD

[4] Clinical Neuroscience Branch, National Institute of Mental Health, Bethesda, MD

来源：

STEROIDS | 1990年 / 55卷 / 07期

关键词：

allopregnanolone; plasma RIA; progesterone; steroids;

D O I：

10.1016/0039-128X(90)90031-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A radioimmunoassay for measuring 3α-hydroxy-5α-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3α-hydroxy-20-oxo-5α-pregnan-11α-yl carboxymethyl ether coupled to bovine serum albumin. 3α-Hydroxy-5α-pregnan-20-one was purified from ether extracts of plasma by high-performance liquid chromatography. These antibodies were then used for the radioimmunoassay of this centrally active progesterone metabolite in rat and human plasma. 3α-Hydroxy-5α-pregnan-20-one was detected in plasma from female rats on the day ofestrus (2.0 to 9.3 ng/ml) and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml. The latter was highly correlated with plasma progesterone levels. © 1990.

引用

页码：290 / 296

页数：7

共 35 条

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