IMMUNE SERUM TO PROTEIN MOLECULAR-WEIGHT STANDARDS FOR CALIBRATING WESTERN BLOTS

被引：7

作者：

CARLONE, GM

PLIKAYTIS, BB

ARKO, RJ

机构：

[1] CTR DIS CONTROL, CTR INFECT DIS, DIV BACTERIAL DIS, IMMUNOL LAB, ATLANTA, GA 30333 USA

[2] CTR DIS CONTROL, CTR INFECT DIS, SEXUALLY TRANSMITTED DIS LAB PROGRAM, ATLANTA, GA 30333 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1986年 / 155卷 / 01期

关键词：

D O I：

10.1016/0003-2697(86)90229-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for calibrating proteins by Western blot analysis is described. Rabbit immune serum, specific for seven protein molecular weight standards, and goat anti-rabbit horseradish peroxidase conjugate are used to visualize standards after electrotransfer onto nitrocellulose. This method can be used as a reagent and procedure control.

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页码：89 / 91

页数：3

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