APPLICATION OF STATISTICAL DESIGN OF EXPERIMENTS TO THE OPTIMIZATION OF FACTOR-VIII EXPRESSION BY CHO CELLS

被引：20

作者：

GANNE, V ^{[1
]}

MIGNOT, G ^{[1
]}

机构：

[1] CTR NATL TRANSFUS SANGUINE,TM INNOVAT,3 AVE TROP,BP 100,F-91943 LES ULIS,FRANCE

来源：

CYTOTECHNOLOGY | 1991年 / 6卷 / 03期

关键词：

CHO CELLS; DOEHLERT MATRIX; EXPERIMENTAL DESIGN; FACTOR-VIII; HADAMARD MATRIX; MATHEMATICAL MODEL; OPTIMIZATION; SERUM-FREE MEDIUM;

D O I：

10.1007/BF00624762

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Selection and optimization of the concentrations of chemically defined components effective on recombinant factor VIII:C production by CHO cells have been performed with statistically designed experiments. Screening of efficient compounds on factor VIII expression was performed by using HADAMARD method, while a precise optimization of the concentrations of two components was achieved with the help of DOEHLERT method. Increased specific activity of factor VIII and reduced cost medium have been obtained with a fewer number of experiments than compared to a less rational approach.

引用

页码：233 / 240

页数：8

共 14 条

[1]

Barnes D., Sato G.H., Methods for growth of cultured cells in serum-free medium, Anal. Biochem., 102, pp. 252-272, (1980)

[2]

Chou H.J.Y., Takahashi S., Control of placental alkaline phosphatase gene expression in Hela cells: induction of synthesis by prednisolone and sodium butyrate, Biochemistry, 26, pp. 3596-3602, (1987)

[3]

De Meo M., Laget M., Phan Tan Luu R., Mathieu D., Dumenil G., Application of experimental design of experiments for media optimization and culture conditions in fermentations, Biosciences, 4, pp. 99-102, (1985)

[4]

Doehlert D.H., Uniform shell designs, Appl. Statistics, 3, pp. 231-239, (1970)

[5]

Dorner A.J., Wisley L.C., Kaufman R.J., Increased synthesis of secreted proteins induces expression of glucoseregulated proteins in butyrate-treated Chinese Ovary Cells, J. Biol. Chem., 264, pp. 20602-20607, (1989)

[6]

Kuosmanen M., Poso H., Inhibition of the activity of restriction endonucleases by spermidine and spermine, FEBS Letters, 179, pp. 17-20, (1985)

[7]

Mignot G., Ganne V., Faure T., Pavirani A., Van De Pol H., The use of a statistical approach for the optimization of culture conditions of genetically engineered cell lines, Advances in Animal Cell Biology and Technology for Bioprocess, pp. 52-58, (1988)

[8]

Murphy T.D., Design and analysis of industrial results, Chemical Engineering, 6, pp. 169-182, (1977)

[9]

Pavirani A., Meulien P., Harrer H., Schamber F., Dott K., Villeval D., Cordier Y., Wiesel M.L., Mazurier C., Van De Pol H., Piquet D., Cazenave J.P., Lecocq J.P., Choosing a host cell for active recombinant factor VIII production using vaccinia virus, Biotechnology, 5, pp. 389-392, (1987)

[10]

Pierce D.A., Fausto N., Mechanism of stimulation of chromatic transcript by putrescine: effects on rate of elongation and number of initiation sites utilized, Biochemistry, 17, pp. 102-109, (1978)

← 1 2 →