MATRIX-ASSISTED UV LASER-DESORPTION OF BIOPOLYMERS - INFLUENCE OF THE INTERACTIONS IN THE TARGET

被引：3

作者：

BLAIS, JC ^{[1
]}

BOLBACH, G ^{[1
]}

RIAHI, K ^{[1
]}

SPIRO, M ^{[1
]}

BRUNOT, A ^{[1
]}

BRETON, F ^{[1
]}

机构：

[1] UNIV PARIS 06,F-75230 PARIS 05,FRANCE

来源：

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE | 1993年 / 90卷 / 06期

关键词：

UV LASER DESORPTION; MATRIX; TIME OF FLIGHT MASS SPECTROMETRY; PROTEINS;

D O I：

10.1051/jcp/1993901399

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of the nature of the matrix and of the macroscopic structure of the target on the MALD spectra of proteins has been systematically studied for matrices such as 2,5-dihydroxybenzoic acid, 3,5-dimethoxy-4-hydroxycinnamic acid, 3-hydroxy-4-methoxycinnamic acid and alpha-cyano-4-hydroxycinnamic acid. Only 3,5-dimethoxy-4-hydroxycinnamic has been found to give homogeneous targets and reproducible spectra. The neutral contribution to the protein molecular and cluster peaks, which depends on the the laser fluence and on the mass of the protein, also strongly depends on the nature of the matrix.

引用

页码：1399 / 1406

页数：8

共 11 条

[1]

BEAVIS R, COMMUNICATION

[2] MATRIX-ASSISTED ULTRAVIOLET-LASER DESORPTION - EVOLUTION AND PRINCIPLES [J].