ANALYSIS OF MADUROSE AND OTHER ACTINOMYCETE WHOLE CELL SUGARS BY GAS-CHROMATOGRAPHY

被引:98
作者
SADDLER, GS [1 ]
TAVECCHIA, P [1 ]
LOCIURO, S [1 ]
ZANOL, M [1 ]
COLOMBO, L [1 ]
SELVA, E [1 ]
机构
[1] MARION MERRELL DOW RES INST,LEPETIT RES CTR,VARESE,ITALY
关键词
ACTINOMYCETE; MADUROSE; SUGAR;
D O I
10.1016/0167-7012(91)90022-I
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The biomass of ActinomadUra madurae, Microtetraspora glauca and Planobispora rosea was hydrolyzed, the released sugars were reduced, acetylated and the resultant alditol acetates analysed by gas chromatography (GC) and GC-mass spectrometry. An unidentified sugar occurring in all strains was confirmed to be madurose (3-O-methyl-D-galactose) by comparison with synthetically prepared madurose which was authenticated by proton nuclear magnetic resonance spectroscopy (H-1-NMR). The occurrence of this sugar is in agreement with current actinomycete taxonomy.
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页码:185 / 191
页数:7
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