CDNA EXPRESSION STUDIES OF RAT-LIVER ARYL SULFOTRANSFERASE

被引:13
作者
CRUICKSHANK, D
SANSOM, LN
VERONESE, ME
MOJARRABI, B
MCMANUS, ME
ZHU, XY
机构
[1] FLINDERS UNIV S AUSTRALIA,SCH MED,DEPT CLIN PHARMACOL,ADELAIDE 5001,AUSTRALIA
[2] UNIV QUEENSLAND,DEPT PHYSIOL & PHARMACOL,QUEENSLAND 4072,AUSTRALIA
关键词
D O I
10.1006/bbrc.1993.1216
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA encoding an isoenzyme of rat liver aryl sulphotransferase was isolated from a rat liver bacteriophage Lambda gt 11 library by the polymerase chain reaction technique. The resulting cDNA was functionally expressed in COS-7 cells and characterised by determining the sulphating capacity of the cells with a range of substrates. The COS-expressed enzyme catalysed the sulphation of both phenol and dopamine with Kms of the same order as those obtained for the high affinity isozyme in rat liver cytosol, while low activity was observed with tyrosine methyl ester. The common food additive vanillin was also a good substrate for sulphate conjugation. The sulphation of vanillin catalysed by the COS-expressed enzyme was consistent with a single enzyme system, in contrast, the kinetics of the reaction catalysed by cytosolic sulphotransferase indicated that vanillin was sulphated by more than one isozyme. © 1993 Academic Press.
引用
收藏
页码:295 / 301
页数:7
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