INDUCTION OF MANGANESE SUPEROXIDE-DISMUTASE MESSENGER-RNA BY OKADAIC ACID AND PROTEIN-SYNTHESIS INHIBITORS

被引：14

作者：

FUJII, J ^{[1
]}

NAKATA, T ^{[1
]}

MIYOSHI, E ^{[1
]}

IKEDA, Y ^{[1
]}

TANIGUCHI, N ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT BIOCHEM,SUITA,OSAKA 565,JAPAN

来源：

BIOCHEMICAL JOURNAL | 1994年 / 301卷

关键词：

D O I：

10.1042/bj3010031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have reported that the phorbol ester phorbol 12-myristate 13-acetate (PMA) enhances the expression of manganese superoxide dismutase (Mn-SOD) mRNA [Fujii and Taniguchi (1991) J. Biol. Chem. 266, 23142-23146]. Okadaic acid, an inhibitor of type I and type IIa phosphatases, was also found to induce Mn-SOD mRNA at submicromolar concentrations in HeLa cells. Addition of cycloheximide resulted in superinduction of PMA-or tumour necrosis factor-stimulated expression of the mRNA, but not of okadaic acid-stimulated expression. When the effect of cycloheximide on the decay of Mn-SOD mRNA was examined by inhibiting mRNA synthesis with actinomycin D, cycloheximide had virtually no effect on mRNA stability, suggesting that accumulation of the mRNA was caused by activation by this reagent of transcription of the gene. PMA pretreatment of HeLa cells markedly enhanced cycloheximide-dependent superinduction of Mn-SOD mRNA. These data suggest that phosphorylation of several proteins is implicated in the regulation of Mn-SOD gene expression.

引用

页码：31 / 34

页数：4

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