Identification of five Rhodobacter capsulatus genes encoding the equivalent of ND subunits of the mitochondrial NADH-ubiquinone oxidoreductase

被引：34

作者：

Dupuis, A

Peinnequin, A

Chevallet, M

Lunardi, J

Darrouzet, E

Pierrard, B

Procaccio, V

Issartel, JP

机构：

[1] CEA, DBMS, CNRS, URA 1130, LAB BIOCHIM, F-38054 GRENOBLE 9, FRANCE

[2] UNIV GRENOBLE 1, JE DRED 119, F-38706 LA TRONCHE, FRANCE

来源：

GENE | 1995年 / 167卷 / 1-2期

关键词：

nqo genes; ndh genes; nuo genes; complex I; NADH-CoQ reductase;

D O I：

10.1016/0378-1119(95)00693-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We previously reported the sequencing of two genes (ndhA and ndhI) encoding two of the subunits of the type-I NADH-ubiquinone oxidoreductase from Rhodobacter capsulatus (Rc). The present paper deals with the cloning and characterization of a chromosomal fragment clustering five new Rc genes which encode subunits of this enzyme. This gene cluster is located immediately downstream from ndhA and ndhI, and also contains two unidentified open reading frames (urf2, urf3). The five genes, nuoJ, nuoK, nuoL, nuoM and nuoN, encode proteins related, respectively, to mitochondrial (mt) subunits ND6, ND4L, ND5, ND4 and ND2. The overall organization of the nuo genes identified in Reshows similarity to that of the Paracoccus denitrificans (Pd) nqo gene cluster.

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页码：99 / 104

页数：6

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