EFFICIENT AMINOACYLATION OF A YEAST TRANSFER RNAASP TRANSCRIPT WITH A 5' EXTENSION

被引：14

作者：

PERRET, V

FLORENTZ, C

GIEGE, R

机构：

[1] Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire, CNRS, 67084 Strasbourg Cedex

来源：

FEBS LETTERS | 1990年 / 270卷 / 1-2期

关键词：

Aminoacylation; Mischarging; Precursor; RNA engineering; tRNA[!sup]Asp[!/sup] transcript;

D O I：

10.1016/0014-5793(90)81221-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A yeast aspartic acid tRNA with a 5' extension of 14 nucleotides was obtained by in vitro transcription with T7 DNA dependent RNA polymerase. This transcript, called extended tRNAAsp transcript, retains its aspartylation capacity with the same Km and only three times reduced kcal values as compared to those measured for canonical tRNAAsp. This result indicates that the 5' extension of the amino acid acceptor stem of tRNAAsp does not interfere with recognition by aspartyl-tRNA synthetase. However, in contrast to the wild-type tRNAAsp transcript, the 5' extended molecule presents a reduced capacity to be mischarged by arginyl-tRNA synthetase, suggesting the existence of different structural requirements in aspartyland arginyl-tRNA synthetases for tRNAAsp recognition. © 1990.

引用

页码：4 / 8

页数：5

共 34 条

[1]

DIRHEIMER G, 1967, B SOC CHIM BIOL, V49, P1679

[2] VALYLATION OF TRANSFER RNA-LIKE TRANSCRIPTS FROM CLONED CDNA OF TURNIP YELLOW MOSAIC-VIRUS RNA DEMONSTRATE THAT THE L-SHAPED REGION AT THE 3' END OF THE VIRAL-RNA IS NOT SUFFICIENT FOR OPTIMAL AMINOACYLATION [J].