DEVELOPMENTAL EXPRESSION OF 2',3'-CYCLIC NUCLEOTIDE 3'-PHOSPHOHYDROLASE IN DISSOCIATED FETAL-RAT BRAIN CULTURES AND RAT-BRAIN

The development in primary dissociated rat brain cultures of 2'',3''-cyclic nucleotide 3''-phosphohydrolase (CNP) activity, the accumulation of CNP protein and the number of cells accumulating this protein were quantitatively determined as a function of time in culture. Parallel determinations were made for the first 2 parameters for developing rat brain. The developmental profile of CNP enzymatic activity and amount of CNP protein in culture paralleled that observed in rat brain, in which the period of most active development occurred 7-25 days after birth. Mean CNP activities of 5.6 and 8.1 .mu.mol/min per mg total protein were recorded for the cultures and rat brain, respectively, at their maximal levels. The corresponding mean values for the CNP protein accumulation were calculated to be 138 and 150 pmol/mg total protein, respectively. Maximal specific activities of the CNP protein were estimated to be .apprx. 800 and 1100 .mu.mol/min per mg CNP protein for culture and rat brain enzyme, respectively; .apprx. 3 million cell expressing CNP apepared in the cultures/dissociated fetal rat brain seeded. Each CNP+ oligodendrocyte in culture had an average CNP activity of 3.2 pmol/min and an average CNP protein content of 0.09 fmol (5.4 .times. 107 molecules), values which remained nearly constant during the course of development. The dissociated fetal brain culture system reproduces rather accurately the temporal developmental pattern of CNP expression occurring in the rat brain, but some important quantitative differences occur which suggest the need for additional environmental stimuli missing in these cultures. The quantitative increases in CNP specific activity and amount of CNP protein occurring during oligodendrocyte differentiation in these cultures are primarily the result of increases in the number of CNP+ cells present which upon differentiation express very quickly, via an off-on regulation, steady-state levels of the enzyme.