TARGETED KILLING OF YEAST EXPRESSING A HIV-1 PEPTIDE BY ANTIBODY-CONJUGATED GLUCOSE-OXIDASE AND HORSERADISH-PEROXIDASE

被引：5

作者：

CHOUCHANE, L

BRINGMAN, T

BARBIER, S

TRAINCARD, F

STROSBERG, AD

机构：

[1] UNIV PARIS 07,IMMUNOPHARMACOL MOLEC LAB,CNRS,F-75221 PARIS 05,FRANCE

[2] IDEON CORP,REDWOOD CITY,CA

[3] CHEMUNEX SA,MAISONS ALFORT,FRANCE

[4] INST PASTEUR,HYBRIDOLAB,F-75724 PARIS 15,FRANCE

来源：

IMMUNOLOGY LETTERS | 1990年 / 25卷 / 04期

关键词：

Antibody-conjugated enzyme; HIV-1; Targeted killing;

D O I：

10.1016/0165-2478(90)90208-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The epitope recognized by monoclonal antibody directed against the HIV-1 recombinant gp160 protein was precisely delineated by using a number of peptides comprising amino acid positions 302-330 of the protein. Two different enzymes, glucose oxidase and horseradish peroxidase, were then coupled to distinct antibody molecules and the efficacy of the immunoenzymes in killing yeast cells which express the recognized peptide was evaluated by flow cytometry analysis. The antibody-glucose oxidase conjugate alone was cytotoxic only at large doses (over 35 μg/ml) while in the presence of the antibody-horseradish peroxidase conjugate, killing was observed at nine times lower concentrations (4 μg/ml). The procedure described here may provide a new immunotherapy tool for microbial infection. © 1990.

引用

页码：359 / 365

页数：7

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