GLUTAMATE AND GABA-MEDIATED SYNAPTIC CURRENTS IN NEURONS OF THE RAT DORSAL MOTOR NUCLEUS OF THE VAGUS

被引:191
作者
TRAVAGLI, RA
GILLIS, RA
ROSSITER, CD
VICINI, S
机构
[1] GEORGETOWN UNIV,MED CTR,SCH MED,FIDIA GEORGETOWN INST NEUROSCI,3900 RESERVOIR RD NW,WASHINGTON,DC 20007
[2] GEORGETOWN UNIV,MED CTR,SCH MED,DEPT PHARMACOL,WASHINGTON,DC 20007
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 260卷 / 03期
关键词
BRAIN STEM SLICES; SPONTANEOUS AND EVOKED SYNAPTIC CURRENTS; WHOLE CELL CLAMP; EXCITATORY AMINO ACID RECEPTORS; GAMMA-AMINOBUTYRIC ACID RECEPTORS;
D O I
10.1152/ajpgi.1991.260.3.G531
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We report the presence of excitatory and inhibitory spontaneous and evoked synaptic currents in the dorsal motor nucleus of the vagus (DMV) in the rat upon vagal and perivagal stimulation. Whole cell current-clamp recordings from anatomically identified DMV neurons in rat brain stem slices show that these neurons are capable of sustained slow-frequency action potential firing probably because of the presence of a pacemaker current. Spontaneously occurring, tetrodotoxin-resistant miniature inhibitory and excitatory synaptic potentials were observed. Stimulation of the vagus mostly induced antidromic action potentials in DMV neurons. However, careful positioning of the stimulating electrode in the tissue surrounding the recording neuron, and sometimes in the vagus itself, was capable of evoking orthodromic-evoked mixed inhibitory-excitatory postsynaptic potentials, and eventually, action potentials. Whole cell voltage-clamp recordings of the synaptic currents corresponding to these synaptic potentials in the presence of pharmacological antagonists of the neurotransmitters gamma-aminobutyric acid (GABA), glutamate, and glycine receptor subtypes indicate that the inhibitory synaptic currents are mediated by GABA-activated Cl- channels, while the excitatory synaptic currents are due to activation of ionotropic gluamate receptors of the N-methyl-D-aspartic acid (NMDA) and non-NMDA subtypes.
引用
收藏
页码:G531 / G536
页数:6
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