REPRESSION OF THE MURINE INTERFERON-ALPHA-11 GENE - IDENTIFICATION OF NEGATIVELY ACTING SEQUENCES

被引：19

作者：

CIVAS, A ^{[1
]}

DION, M ^{[1
]}

VODJDANI, G ^{[1
]}

DOLY, J ^{[1
]}

机构：

[1] INST RECH SCI CANC,CNRS,UPR 37,BP 8,F-94800 VILLEJUIF,FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 16期

关键词：

D O I：

10.1093/nar/19.16.4497

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The uninducible murine interferon alpha-11 gene (Mu IFN-alpha-11) shows strong homology with the highly inducible Mu IFN-alpha-4 gene in the promoter region. Negative regulatory sequences located between positions -470 and -145 were characterized in the Mu IFN-alpha-11 promoter. The removal of these sequences leads to virus-inducibility of Mu IFN-alpha-11 while their insertion in Mu IFN-alpha-4 corresponding region significantly reduced the inducibility of Mu IFN-alpha-4 promoter. On the other hand, the virus-responsive element (VRE) of the Mu IFN-alpha-11 differs by a single nucleotide substitution at position -78 from the VRE-alpha-4. Constructions carrying either VRE-alpha-11 or VRE-alpha-4 upstream a heterologous promoter displayed different virus inducibilities. The -78 A/G substitution affects the inducibility by decreasing the affinity of VRE-binding trans-regulators. Our results suggest that the combined effect of the negative regulatory sequences and of the mutation in the VRE-alpha-11, completely silences the Mu IFN-alpha-11 gene.

引用

页码：4497 / 4502

页数：6

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