CYCLIC ADP-RIBOSE-INDUCED CA2+ RELEASE FROM RAT-BRAIN MICROSOMES

被引：105

作者：

WHITE, AM

WATSON, SP

GALIONE, A

机构：

[1] Department of Pharmacology, University of Oxford, Oxford

来源：

FEBS LETTERS | 1993年 / 318卷 / 03期

关键词：

CYCLIC ADP-RIBOSE; CALCIUM; RAT BRAIN MICROSOME; RYANODINE RECEPTOR;

D O I：

10.1016/0014-5793(93)80524-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclic ADP-ribose (cADPR), an endogenous NAD+ metabolite in many mammalian and invertebrate tissues, is a potent mediator of calcium mobilization in sea urchin eggs. Our results show that cADPR also stimulates calcium release from rat brain microsomes, marked release occurring over the concentration range 10-250 nM. This is not inhibited by concentrations of heparin which completely abolish inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release. Ryanodine (100 muM) inhibits the cADPR response. Our results are consistent with cADPR being an endogenous messenger mediating Ca2+ release from ryanodine-sensitive pools in brain.

引用

页码：259 / 263

页数：5

共 17 条

[1] ACTIVATION AND CONDUCTANCE PROPERTIES OF RYANODINE-SENSITIVE CALCIUM CHANNELS FROM BRAIN MICROSOMAL-MEMBRANES INCORPORATED INTO PLANAR LIPID BILAYERS [J].