COMPARISON OF 16S RIBOSOMAL-RNA GENES IN CLAVIBACTER-MICHIGANENSIS SUBSPECIES WITH OTHER CORYNEFORM BACTERIA

被引：21

作者：

LI, X

DEBOER, SH

机构：

[1] Agriculture Canada, Pacific Agriculture Research Centre, Vancouver

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1995年 / 41卷 / 10期

关键词：

CLAVIBACTER; CORYNEFORM BACTERIA; PHYLOGENY; 16S RIBOSOMAL-RNA ANALYSIS;

D O I：

10.1139/m95-127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nearly complete sequences (97-99%) of the 16S rRNA genes were determined for type strains of Clavibacter michiganensis subsp. michiganensis, Clavibacter michiganensis subsp. insidiosus, Clavibacter michiganensis subsp. sepedonicus, and Clavibacter michiganensis subsp. nebraskensis. The four subspecies had less than 1% dissimilarity in their 16S rRNA genes. Comparative studies indicated that the C. michiganensis subsp. shared relatively high homology with the 16S rRNA gene of Clavibacter xyli. Further comparison with representatives of other Gram-positive coryneform and related bacteria with high G+C% values showed that this group of bacteria was subdivided into three clusters. One cluster consisted of the Clavibacter michiganensis subsp., Clavibacter xyli, Arthrobacter globiformis, Arthrobacter simplex, and Frankia sp.; another cluster consisted of members of the corynebacteria-mycobacteria-nocardia (CMN) group of Mycobacteriaceae including Tsukamurella paur ometabolum; and Propionibacterium freudenreichii alone formed a unique cluster, which was remote from other coryneform bacteria analyzed. The three clusters may reflect a systematic rank higher than the genus level among these bacteria.

引用

页码：925 / 929

页数：5

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