IMPROVED CULTURE CONDITIONS FOR COWDRIA-RUMINANTIUM (RICKETTSIALES), THE AGENT OF HEARTWATER DISEASE OF DOMESTIC RUMINANTS

被引：51

作者：

BYROM, B

YUNKER, CE

机构：

[1] University of Florida/U.S. Agency for International Development/Zimbabwe Heartwater Research Project, Causeway

来源：

CYTOTECHNOLOGY | 1990年 / 4卷 / 03期

关键词：

HEARTWATER; CELL CULTURE; COWDRIA-RUMINANTIUM; BOVINE VASCULAR ENDOTHELIAL CELLS;

D O I：

10.1007/BF00563789

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The causal agent of heartwater disease of domestic ruminants, Cowdria ruminantium, can, with difficulty, be isolated and passaged in lines of bovine endothelial cells grown in the presence of the Glasgow modification of Eagle's minimal essential medium. However, when Leibovitz's L-15 medium supplemented with 0.45% glucose at pH 6.0-6.5 is used as a maintenance medium for these cells, isolation and serial passage may routinely be achieved.

引用

页码：285 / 290

页数：6

共 13 条

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