DETECTION OF ROTAVIRUS IN SOUTH-AFRICAN WATERS - A COMPARISON OF A CYTOIMMUNOLABELLING TECHNIQUE WITH COMMERCIALLY AVAILABLE IMMUNOASSAYS

被引：8

作者：

GENTHE, B ^{[1
]}

IDEMA, GK ^{[1
]}

KFIR, R ^{[1
]}

GRABOW, WOK ^{[1
]}

机构：

[1] UNIV PRETORIA,DEPT MED VIROL,PRETORIA,SOUTH AFRICA

来源：

WATER SCIENCE AND TECHNOLOGY | 1991年 / 24卷 / 02期

关键词：

ROTAVIRUS; CYTOIMMUNOPEROXIDASE; ELISA; LATEX AGGLUTINATION; MA104; CELLS; MARINE WATER; RIVER WATER; WASTE-WATER EFFLUENT; GASTROENTERITIS; DIARRHEA; SEWAGE;

D O I：

10.2166/wst.1991.0066

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A cytoimmunolabelling technique was compared with commercially available immunoassays for the detection of the simian rotavirus SA11 and human rotavirus in various environmental samples. The technique is based on labelling MA104 cells with antibody conjugated with an enzyme for the detection of rotavirus. Water samples were concentrated by ultrafiltration and inoculated on trypsin-treated MA104 cells. After 18 h incubation, evidence of viral replication was determined by immunolabelling of viral antigen using antibodies tagged with horseradish peroxidase. The cytoimmunolabelling method was shown to be 10(5) times more sensitive for the detection of rotavirus than commercially available ELISA and latex agglutination kits. The cytoimmunolabelling technique was also shown to be successful for the detection of human rotavirus in river and marine waters and wastewater effluents.

引用

页码：241 / 244

页数：4

共 10 条

[1] COMPARISON OF DIRECT ELECTRON-MICROSCOPY, IMMUNE ELECTRON-MICROSCOPY, AND ROTAVIRUS ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR DETECTION OF GASTROENTERITIS VIRUSES IN CHILDREN [J].