SCREENING FOR NASOPHARYNGEAL CARCINOMA WITH AN ELISA USING THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN, EBNA-1 - A COMPLEMENTARY TEST TO THE IGA/VCA IMMUNOFLUORESCENCE ASSAY

被引：12

作者：

CHENG, HM ^{[1
]}

FOONG, YT ^{[1
]}

MATHEW, A ^{[1
]}

SAM, CK ^{[1
]}

DILLNER, J ^{[1
]}

PRASAD, U ^{[1
]}

机构：

[1] KAROLINSKA INST,DEPT VIROL,S-10401 STOCKHOLM 60,SWEDEN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 42卷 / 01期

关键词：

EBNA-1; PEPTIDE; NASOPHARYNGEAL CARCINOMA;

D O I：

10.1016/0166-0934(93)90175-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An ELISA using the Epstein-Barr virus nuclear antigen 1 (EBNA 1) was found to detect selectively specific IgA in sera from patients with nasopharyngeal carcinoma (NPC). The antigen, p107, was a 20-amino acid synthetic peptide, representing a major epitope of EBNA 1. 267/294 (90.8%) of NPC patients had IgA antibodies to p107 but in normal individuals, only 41/577 (7.1%) had IgA/p107. In sera from patients with other cancers, 11/77 (14.3%) had IgA/p107 reactivity. 124 IgA/VCA positive and 86 IgA/VCA negative NPC sera were also tested for IgA/p107 binding in ELISA. The majority of IgA/VCA positive sera (117) also contained IgA/p107 antibodies. Of interest was the detection of 74/86 IgA/p107 reactive sera in the IgA/VCA negative group. The results suggest that the IgA/p107 ELISA could become a useful, complementary screening assay to the IgA/VCA immunofluorescence test for detection of NPC.

引用

页码：45 / 52

页数：8

共 17 条

[1] CROSS REACTION OF ANTIBODIES TO A GLYCINE ALANINE REPEAT SEQUENCE OF EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-1 WITH COLLAGEN, CYTOKERATIN, AND ACTIN [J].

BABOONIAN, C ;

VENABLES, PJW ;

WILLIAMS, DG ;

WILLIAMS, RO ;

MAINI, RN .

ANNALS OF THE RHEUMATIC DISEASES, 1991, 50 (11) :772-775

[2] EPSTEIN-BARR-VIRUS LATENT GENE-TRANSCRIPTION IN NASOPHARYNGEAL CARCINOMA-CELLS - COEXPRESSION OF EBNA1, LMP1, AND LMP2 TRANSCRIPTS [J].

BROOKS, L ;

YAO, QY ;

RICKINSON, AB ;

YOUNG, LS .

JOURNAL OF VIROLOGY, 1992, 66 (05) :2689-2697

[3] CONSISTENT TRANSCRIPTION OF THE EPSTEIN-BARR-VIRUS LMP2 GENE IN NASOPHARYNGEAL CARCINOMA [J].

BUSSON, P ;

MCCOY, R ;

SADLER, R ;

GILLIGAN, K ;

TURSZ, T ;

RAABTRAUB, N .

JOURNAL OF VIROLOGY, 1992, 66 (05) :3257-3262

[4] EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-1 LINEAR EPITOPES THAT ARE REACTIVE WITH IMMUNOGLOBULIN A (IGA) OR IGG IN SERA FROM NASOPHARYNGEAL CARCINOMA PATIENTS OR FROM HEALTHY DONORS [J].

CHENG, HM ;

FOONG, YT ;

SAM, CK ;

PRASAD, U ;

DILLNER, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (10) :2180-2186

[5] A SYNTHETIC PEPTIDE DEFINES A SEROLOGIC IGA RESPONSE TO A HUMAN PAPILLOMAVIRUS-ENCODED NUCLEAR ANTIGEN EXPRESSED IN VIRUS-CARRYING CERVICAL NEOPLASIA [J].

DILLNER, J ;

DILLNER, L ;

ROBB, J ;

WILLEMS, J ;

JONES, I ;

LANCASTER, W ;

SMITH, R ;

LERNER, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3838-3841

[6] ANTIBODIES AGAINST A SYNTHETIC PEPTIDE IDENTIFY THE EPSTEIN-BARR VIRUS-DETERMINED NUCLEAR ANTIGEN [J].

DILLNER, J ;

STERNAS, L ;

KALLIN, B ;

ALEXANDER, H ;

EHLINHENRIKSSON, B ;

JORNVALL, H ;

KLEIN, G ;

LERNER, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (15) :4652-4656

[7] THE EPSTEIN-BARR VIRUS PROTEINS [J].

DILLNER, J ;

KALLIN, B .

ADVANCES IN CANCER RESEARCH, 1988, 50 :95-158

[8] EXPRESSION OF EPSTEIN-BARR VIRUS-ENCODED PROTEINS IN NASOPHARYNGEAL CARCINOMA [J].

FAHRAEUS, R ;

FU, HL ;

ERNBERG, I ;

FINKE, J ;

ROWE, M ;

KLEIN, G ;

FALK, K ;

NILSSON, E ;

YADAV, M ;

BUSSON, P ;

TURSZ, T ;

KALLIN, B .

INTERNATIONAL JOURNAL OF CANCER, 1988, 42 (03) :329-338

[9] SERUM AND SALIVARY IGA ANTIBODIES AGAINST A DEFINED EPITOPE OF THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN (EBNA) ARE ELEVATED IN NASOPHARYNGEAL CARCINOMA [J].

FOONG, YT ;

CHENG, HM ;

SAM, CK ;

DILLNER, J ;

HINDERER, W ;

PRASAD, U .

INTERNATIONAL JOURNAL OF CANCER, 1990, 45 (06) :1061-1064

[10]

GUILBERT B, 1982, J IMMUNOL, V128, P2779

← 1 2 →