A SILVER IMPREGNATION METHOD FOR LABELING BOTH ALZHEIMER PAIRED HELICAL FILAMENTS AND THEIR POLYPEPTIDES SEPARATED BY SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS

被引：51

作者：

IQBAL, K ^{[1
]}

BRAAK, E ^{[1
]}

BRAAK, H ^{[1
]}

ZAIDI, T ^{[1
]}

GRUNDKEIQBAL, I ^{[1
]}

机构：

[1] ZENTRUM MORPHOL,W-600 FRANKFURT 70,GERMANY

来源：

NEUROBIOLOGY OF AGING | 1991年 / 12卷 / 04期

关键词：

ALZHEIMER DISEASE; GALLYAS SILVER STAIN; MICROTUBULE-ASSOCIATED PROTEINS; NEUROFILAMENT TRIPLET; PAIRED HELICAL FILAMENTS; SILVER IMPREGNATION; TAU-PROTEIN; TUBULIN; UBIQUITIN;

D O I：

10.1016/0197-4580(91)90022-C

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

The Gallyas silver impregnation which is specific to neurofibrillary changes of paired helical filaments (PHF) and 15 nn straight filaments, was adapted to stain polypeptides separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both PHF and tau polypeptides were readily and consistently stained by the Gallyas stain. This technique stained PHF > tau > high-molecular-weight microtubule-associated polypeptides (MAPS). Tubulin was stained only weakly. Neurofilament triplet, ubiquitin, bovine serum albumin and histones were unstained. The staining of PHF and tau polypeptides by Gallyas silver stain is consistent with the presence of tau in PHF.

引用

页码：357 / 361

页数：5

共 30 条

[1] NEUROFIBRILLARY TANGLES IN ALZHEIMERS-DISEASE AND PROGRESSIVE SUPRANUCLEAR PALSY - ANTIGENIC SIMILARITIES AND DIFFERENCES - MICROTUBULE-ASSOCIATED PROTEIN-TAU ANTIGENICITY IS PROMINENT IN ALL TYPES OF TANGLES [J].