TROPANE ALKALOIDS IN THE ADVENTITIOUS AND HAIRY ROOT CULTURES OF SOLANACEOUS PLANTS

被引：48

作者：

SHIMOMURA, K ^{[1
]}

SAUERWEIN, M ^{[1
]}

ISHIMARU, K ^{[1
]}

机构：

[1] SAGA UNIV,FAC AGR,GENET ENGN LAB,SAGA 840,JAPAN

来源：

PHYTOCHEMISTRY | 1991年 / 30卷 / 07期

关键词：

DATURA-INNOXIA; DUBOISIA HYBRID; HYOSCYAMUS-ALBUS; H-NIGER; SCOPOLIA-TANGUTICA; SOLANACEAE; ADVENTITIOUS ROOT CULTURE; HAIRY ROOT CULTURE; TROPANE ALKALOID;

D O I：

10.1016/0031-9422(91)83629-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adventitious and hairy root cultures of several solanaceous plant species were established. The highest content of tropane alkaloids was obtained in the adventitious roots of Hyoscyamus albus cultured in hormone-free 1/2 MS liquid medium. On the other hand the hairy roots of H. albus transformed with A. rhizogenes strain MAFF 03-01724 grew very fast and yielded over 5 mg/100 ml flask of hyoscyamine within 22 days of culture. The alkaloid content in the hairy roots of H. niger was higher than that of its adventitious roots. 7-beta-Hydroxyhyoscyamine, recently isolated, was produced in the hairy roots of Scopolia tangutica and Datura innoxia, while it could not be detected in their adventitious root cultures.

引用

页码：2275 / 2278

页数：4

共 14 条

[1] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].