SIMPLIFICATION OF PROCEDURES USED TO TEST URINE SAMPLES FOR CHLAMYDIA TRACHOMATIS

被引:17
作者
THOMAS, BJ [1 ]
GILCHRIST, C [1 ]
HAY, PE [1 ]
TAYLORROBINSON, D [1 ]
机构
[1] ST MARYS HOSP,LONDON W2 1NY,ENGLAND
关键词
D O I
10.1136/jcp.44.5.374
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Two methods of simplifying the procedure for examining urine samples for Chlamydia trachomatis were investigated. When 73 urine samples from 56 men with acute non-gonococcal urethritis were examined by direct immunofluorescence (Micro Trak), centrifuging 1 ml volumes of urine at 13 000 rpm for five minutes was at least as efficient for detecting C trachomatis as centrifuging larger volumes at 3000 rpm for 30 minutes. Furthermore, examination of urine produced during a visit to a sexually transmitted disease clinic was at least as efficient as examination of early morning urine for detecting C trachomatis by Micro Trak, or by an enzyme immunoassay (IDEIA). Both modifications have practical advantages and should encourage the use of urine samples for diagnosing chlamydial infections in men.
引用
收藏
页码:374 / 375
页数:2
相关论文
共 6 条
[1]  
CAUL EO, 1988, LANCET, V2, P1246
[2]  
HAY PE, 1991, IN PRESS GENITOURIN
[3]  
MATTHEWS RS, 1989, LANCET, V1, P96
[4]   EVALUATION OF 3 CHLAMYDIA-TRACHOMATIS IMMUNOASSAYS WITH AN UNBIASED, NONINVASIVE CLINICAL-SAMPLE [J].
PAUL, ID ;
CAUL, EO .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (02) :220-222
[5]   CHLAMYDIA-TRACHOMATIS URETHRAL INFECTIONS IN MEN [J].
STAMM, WE ;
KOUTSKY, LA ;
BENEDETTI, JK ;
JOURDEN, JL ;
BRUNHAM, RC ;
HOLMES, KK .
ANNALS OF INTERNAL MEDICINE, 1984, 100 (01) :47-51
[6]   SENSITIVITY OF DETECTING CHLAMYDIA-TRACHOMATIS ELEMENTARY BODIES IN SMEARS BY USE OF A FLUORESCEIN LABELED MONOCLONAL-ANTIBODY - COMPARISON WITH CONVENTIONAL CHLAMYDIAL ISOLATION [J].
THOMAS, BJ ;
EVANS, RT ;
HAWKINS, DA ;
TAYLORROBINSON, D .
JOURNAL OF CLINICAL PATHOLOGY, 1984, 37 (07) :812-816