CONSERVATION IN HUMAN AND MOUSE PUR-ALPHA OF A MOTIF COMMON TO SEVERAL PROTEINS INVOLVED IN INITIATION OF DNA-REPLICATION

被引：96

作者：

MA, ZW

BERGEMANN, AD

JOHNSON, EM

机构：

[1] CUNY MT SINAI SCH MED,DEPT PATHOL,NEW YORK,NY 10029

[2] CUNY MT SINAI SCH MED,BROOKDALE CTR MOLEC BIOL,NEW YORK,NY 10029

来源：

GENE | 1994年 / 149卷 / 02期

关键词：

AMPHIPATHIC HELIX; MCM PROTEINS; SV40; T-ANTIGEN; RETINOBLASTOMA PROTEIN; RB; PURA; PURB;

D O I：

10.1016/0378-1119(94)90167-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Pur alpha is a sequence-specific single-stranded DNA-binding protein with affinity for an element present in several eukaryotic origins of DNA replication (ori) and gene regulatory regions. We report here the cDNA sequence for mouse pur alpha and an extraordinary degree of conservation between human and mouse Pur alpha (hPurA and mPurA, respectively). There are only two single-amino-acid (aa) changes between hPurA (322 aa) and mPurA (321 aa). One PurA region of 22 aa, termed the 'psyche' motif, possesses significant homology to a counterpart in the SV40 large T-antigen, to several other transforming proteins of DNA tumor viruses, and to certain cellular proteins in yeast and human cells that may also be involved in the initiation of DNA replication.

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页码：311 / 314

页数：4

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