INHIBITION OF PLANT ORNITHINE AMINOTRANSFERASE BY GABACULINE AND 4-AMINO-5-HEXYNOIC ACID

被引：6

作者：

HERVIEU, F ^{[1
]}

LEDILY, F ^{[1
]}

LESAOS, J ^{[1
]}

BILLARD, JP ^{[1
]}

HUAULT, C ^{[1
]}

机构：

[1] UNIV CAEN,INST RECH BIOL APPL,INRA,PHYSIOL & BIOCHIM VEGETALES LAB,F-14032 CAEN,FRANCE

来源：

PHYTOCHEMISTRY | 1993年 / 34卷 / 05期

关键词：

RAPHANUS-SATIVUS; BRASSICACEAE; ORNITHINE METABOLISM; ORNITHINE AMINOTRANSFERASE; GABACULINE; 4-AMINO-5-HEXYNOIC ACID;

D O I：

10.1016/0031-9422(91)80006-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gabaculine (GAB) and 4-amino-5-hexynoic acid (AHA) have been shown to be enzyme-activated inhibitors of radish ornithine aminotransferase (OAT). The in vitro results were entirely compatible with the in vivo situation. However, it appeared that GAB was a more effective inhibitor than AHA as it was active at a lower concentration (1 muM) leading in vivo to almost complete irreversible inhibition of OAT within a few hours. GAB and AHA will be useful tools to study the physiological consequences of OAT inhibition in plants.

引用

页码：1231 / 1234

页数：4

共 18 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] MECHANISM OF INACTIVATION AND IDENTIFICATION OF SITES OF MODIFICATION OF ORNITHINE AMINOTRANSFERASE BY 4-AMINOHEX-5-YNOATE [J].

DEBIASE, D ;

SIMMACO, M ;

BARRA, D ;

BOSSA, F ;

HEWLINS, M ;

JOHN, RA .

BIOCHEMISTRY, 1991, 30 (08) :2239-2246

[3]

DEMAISON AM, 1983, PLANT PHYSIOL, V81, P692

[4] ARGININE CATABOLISM IN THE COTYLEDONS OF DEVELOPING AND GERMINATING PEA-SEEDS [J].

DERUITER, H ;

KOLLOFFEL, C .

PLANT PHYSIOLOGY, 1983, 73 (03) :525-528

[5] A BIFUNCTIONAL ENZYME (DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHETASE) CATALYZES THE 1ST 2 STEPS IN PROLINE BIOSYNTHESIS IN PLANTS [J].

HU, CAA ;

DELAUNEY, AJ ;

VERMA, DPS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) :9354-9358

[6] CONVERSION OF GLUTAMATE TO ORNITHINE AND PROLINE - PYRROLINE-5-CARBOXYLATE, A POSSIBLE MODULATOR OF ARGININE REQUIREMENTS [J].

JONES, ME .

JOURNAL OF NUTRITION, 1985, 115 (04) :509-515

[7]

JUNG MJ, 1978, J BIOL CHEM, V253, P7431

[8]

KANDPAL RP, 1982, BIOCHEM INT, V5, P297

[9]

KIRYAN IG, 1984, SOV PLANT PHYSIOL+, V31, P561

[10]

LEDILY F, 1993, IN PRESS PHYSL PLANT

← 1 2 →