CRYSTAL-STRUCTURE OF CASEIN KINASE-1, A PHOSPHATE-DIRECTED PROTEIN-KINASE

The structure of a truncated variant of casein kinase-1 from Schizosacckaromyces pombe, has been determined in complex with MgATP at 2.0 Angstrom resolution. The model resembles the 'closed', ATP-bound conformations of the cyclin-dependent kinase 2 and the cAMP-dependent protein kinase, with clear differences in the structure of surface loops that impart unique features to casein kinase-1. The structure is of unphosphorylated, active conformation of casein kinase-1 and the peptide-binding site is fully accessible to substrate.