PHOSPHORYLATION OF SMALL MOLECULAR-WEIGHT POLYPEPTIDES IN THE IRIS-CILIARY COMPLEX, AQUEOUS-HUMOR AND VITREOUS-HUMOR

被引：5

作者：

BANERJEE, A ^{[1
]}

RICHIERT, DM ^{[1
]}

BAGCHI, M ^{[1
]}

机构：

[1] WAYNE STATE UNIV,SCH MED,DEPT ANAT & CELL BIOL,DETROIT,MI 48201

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1077卷 / 01期

关键词：

PHOSPHORYLATION; VITREOUS HUMOR; IRIS-CILIARY COMPLEX; TISSUE CULTURE; GROWTH INHIBITION;

D O I：

10.1016/0167-4838(91)90525-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polypeptides of vitreous humor, aqueous humor and iris-ciliary complex cells of eyes were phosphorylated with [gamma-P-32]ATP without exogenous protein kinase. Phosphorylated polypeptides were analyzed by sodium dodecyl sulfate gel electrophoresis and autoradiography. The phosphorylated polypeptides of rabbit vitreous humor showed many high molecular weight prominent bands, but no detectable phosphoproteins were found in the 12 kDa or lower range. Bovine vitreous humor has predominantly acidic polypeptides and some of them are below 20 kDa. Rabbit and bovine iris-ciliary complex and rabbit aqueous humor showed a prominent common 4 kDa phosphopolypeptide which could also be synthesized by cloned populations of cells from the bovine iris and the rabbit iris-ciliary body. It is possible that the 4 kDa phosphopolypeptide of the aqueous humor is synthesized by the iris-ciliary complex cells.

引用

页码：56 / 64

页数：9

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