EFFECT OF CA2+ ON THE CRYOPROTECTIVE ACTION OF TREHALOSE

被引：30

作者：

BAKAS, LS

DISALVO, EA

机构：

[1] Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900 La Plata, Sucursal 4

来源：

CRYOBIOLOGY | 1991年 / 28卷 / 04期

关键词：

D O I：

10.1016/0011-2240(91)90041-L

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The competitive effect of Ca2+ on the cryoprotective action of carbohydrates has been investigated during freeze-thaw processes of unilamellar egg phosphatidylcholine vesicles. Ca2+ inhibits the cryoprotection achieved by trehalose to a greater extent than other sugars such as galactose, sucrose, and fructose. The cryoprotection by trehalose is also dependent on the Ca2+ concentration in the inside solution of the vesicle, even in the absence of external Ca2+. The competitive effect of Ca2+/trehalose is interpreted as a consequence of the different amount of interfacial water displaced by each compound in their adsorption on the water/lipid interface. © 1991.

引用

页码：347 / 353

页数：7

共 35 条

[1] EFFECT OF CARBOHYDRATES AND GLYCEROL ON THE STABILITY AND SURFACE-PROPERTIES OF LYOPHILIZED LIPOSOMES [J].

ALONSOROMANOWSKI, S ;

BIONDI, AC ;

DISALVO, EA .

JOURNAL OF MEMBRANE BIOLOGY, 1989, 108 (01) :1-11

[2] CRYOPRESERVATION OF SPERM FROM THE MARINE SHRIMP SICYONIA-INGENTIS [J].

ANCHORDOGUY, T ;

CROWE, JH ;

GRIFFIN, FJ ;

CLARK, WH .

CRYOBIOLOGY, 1988, 25 (03) :238-243

[3] MODES OF INTERACTION OF CRYOPROTECTANTS WITH MEMBRANE PHOSPHOLIPIDS DURING FREEZING [J].

ANCHORDOGUY, TJ ;

RUDOLPH, AS ;

CARPENTER, JF ;

CROWE, JH .

CRYOBIOLOGY, 1987, 24 (04) :324-331

[4] NO PHOSPHOLIPID MONOLAYER SUGAR INTERACTIONS [J].

ARNETT, EM ;

HARVEY, N ;

JOHNSON, EA ;

JOHNSTON, DS ;

CHAPMAN, D .

BIOCHEMISTRY, 1986, 25 (18) :5239-5242

[5] EFFECT OF THE ASYMMETRIC CA-2+ DISTRIBUTION ON THE BILAYER PROPERTIES OF PHOSPHATIDYLCHOLINE-SONICATED VESICLES [J].

BAKAS, LS ;

DISALVO, EA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 979 (03) :352-360

[6]

BAKAS LS, IN PRESS CRYOBIOLOGY

[7] NMR STUDIES ON WATER IMMOBILIZATION BY LIPID SYSTEMS IN VITRO AND IN VIVO [J].

CERBON, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 144 (01) :1-&

[8] TREHALOSE AS CRYOPROTECTANT FOR PRESERVATION OF YEAST STRAINS [J].

COUTINHO, C ;

BERNARDES, E ;

FELIX, D ;

PANEK, AD .

JOURNAL OF BIOTECHNOLOGY, 1988, 7 (01) :23-32

[9] PRESERVATION OF MEMBRANES IN ANHYDROBIOTIC ORGANISMS - THE ROLE OF TREHALOSE [J].

CROWE, JH ;

CROWE, LM ;

CHAPMAN, D .

SCIENCE, 1984, 223 (4637) :701-703

[10] EFFECTS OF CARBOHYDRATES ON MEMBRANE STABILITY AT LOW WATER ACTIVITIES [J].

CROWE, LM ;

MOURADIAN, R ;

CROWE, JH ;

JACKSON, SA ;

WOMERSLEY, C .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 769 (01) :141-150

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