HUMAN UROKINASE CONTAINS GALNAC-BETA(1-4)[FUC-ALPHA(1-3)]GLCNAC-BETA(1-2) AS A NOVEL TERMINAL ELEMENT IN N-LINKED CARBOHYDRATE CHAINS

被引：54

作者：

BERGWERFF, AA

THOMASOATES, JE

VANOOSTRUM, J

KAMERLING, JP

VLIEGENTHART, JFG

机构：

[1] UNIV UTRECHT,BIJVDET CTR,DEPT BIOORGAN CHEM,POB 80075,3508 TB UTRECHT,NETHERLANDS

[2] UNIV UTRECHT,DEPT MASS SPECTROMETRY,UTRECHT,NETHERLANDS

[3] CIBA GEIGY AG,DEPT BIOTECHNOL,CH-4002 BASEL,SWITZERLAND

来源：

FEBS LETTERS | 1992年 / 314卷 / 03期

关键词：

UROKINASE; PLASMINOGEN ACTIVATOR; N-LINKED CARBOHYDRATE CHAIN; FAST ATOM BOMBARDMENT MASS SPECTROSCOPY (FAB-MS); H-1 NMR SPECTROSCOPY;

D O I：

10.1016/0014-5793(92)81512-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structural analysis of enzymically released N-linked carbohydrate chains of human urokinase (urinary-type plasminogen activator) by H-1 NMR spectroscopy and FAB-MS demonstrated that the N-linked oligosaccharides on the only N-glycosylation site contain diantennary structures with the novel GalNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-2) element in the upper or the lower branch.

引用

页码：389 / 394

页数：6

共 29 条

[1] MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY
BAX, A
DAVIS, DG
[J]. JOURNAL OF MAGNETIC RESONANCE, 1985, 65 (02) : 355 - 360
[2] CHARACTERIZATION OF A POSTTRANSLATIONAL FUCOSYLATION IN THE GROWTH-FACTOR DOMAIN OF URINARY PLASMINOGEN-ACTIVATOR
BUKO, AM
KENTZER, EJ
PETROS, A
MENON, G
ZUIDERWEG, ERP
SARIN, VK
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) : 3992 - 3996
[3] CHAN A L, 1991, Glycobiology, V1, P173, DOI 10.1093/glycob/1.2.173
[4] A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES
CIUCANU, I
KEREK, F
[J]. CARBOHYDRATE RESEARCH, 1984, 131 (02) : 209 - 217
[5] DAMM JBL, 1989, RECL TRAV CHIM PAY B, V108, P351
[6] PLASMINOGEN ACTIVATORS, TISSUE DEGRADATION, AND CANCER
DANO, K
ANDREASEN, PA
GRONDAHLHANSEN, J
KRISTENSEN, P
NIELSEN, LS
SKRIVER, L
[J]. ADVANCES IN CANCER RESEARCH, 1985, 44 : 139 - 266
[7] FAB-MASS SPECTROMETRY OF CARBOHYDRATES
DELL, A
[J]. ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1987, 45 : 19 - 72
[8] CARBOHYDRATE DIFFERENTIATION ANTIGENS - PROBABLE LIGANDS FOR CELL-ADHESION MOLECULES
FEIZI, T
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1991, 16 (03) : 84 - 86
[9] FUKUDA M, 1984, J BIOL CHEM, V259, P8260
[10] GREEN ED, 1985, J BIOL CHEM, V260, P5623

← 1 2 3 →