AN EFFICIENT METHOD FOR THE ISOLATION OF INTRAMUSCULAR COLLAGEN

Ultrasonic fragmentation of the myofibrils and subsequent solubilisation in buffer has been used to isolate intramuscular collagen (IMC) in high yield and purity. The method is superior to previously reported techniques in providing both a high yield of collagen and intact fibres. The material obtained is suitable for both physical and biochemical analysis in attempts to demonstrate its role in determining the tenderness of meat.