HIGH MOBILITY GROUP CHROMOSOMAL-PROTEINS BIND TO AT-RICH TRACTS FLANKING PLANT GENES

被引:101
作者
PEDERSEN, TJ
ARWOOD, LJ
SPIKER, S
GUILTINAN, MJ
THOMPSON, WF
机构
[1] N CAROLINA STATE UNIV,DEPT BOT,RALEIGH,NC 27695
[2] N CAROLINA STATE UNIV,DEPT GENET,RALEIGH,NC 27695
关键词
AT-RICH SEQUENCES; DNA-BINDING PROTEINS; FERREDOXIN-I; HMG; HIGH MOBILITY GROUP PROTEINS;
D O I
10.1007/BF00017920
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
AT-rich sequences in the 5' flanking regions of several plant genes have been shown to bind nuclear proteins, but the nature of these proteins has remained largely unknown. We report here that certain plant high mobility group (HMG) chromosomal proteins can interact specifically (in the presence of excess non-specific competitor) with AT-rich sequences located upstream of the pea ferredoxin 1 gene (Fed-1) and a member of the wheat Em gene family. Binding was observed with highly purified preparations of HMGa or HMGb, but not with HMGc or HMGd. HMG-DNA complexes were similar to one of the two types of Fed-1 complexes we observed previously using pea nuclear extracts [7]. HMG binding to the Fed-1 DNA was localized to a region containing AT-rich sequences; very similar sequences are present 5' to Em and several other plants genes. Such sequences have been shown to bind unidentified nuclear proteins in a number of these systems. Binding experiments with a synthetic oligo (dA). oligo (dT) probe and competition experiments with synthetic DNA polymers suggest that HMG binding may depend upon structural features of AT-rich DNA rather than being sequence-specific. We discuss the implications of these findings and suggest a role for HMG binding which is consistent with previous evidence linking HMGs with transcriptionally competent chromatin.
引用
收藏
页码:95 / 104
页数:10
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