SPERM NUCLEI ANALYSIS OF A ROBERTSONIAN T(14Q21Q) CARRIER, BY FISH, USING 3 PLASMIDS AND 2 YAC PROBES

被引：69

作者：

ROUSSEAUX, S

CHEVRET, E

MONTEIL, M

COZZI, J

PELLETIER, R

DELAFONTAINE, D

SELE, B

机构：

[1] GRENOBLE UNIV,SCH MED,ALBERT BONNIOT INST,DYOGEN,REPROD BIOL UNIT,F-38706 LA TRONCHE,FRANCE

[2] BOUCICAULT HOSP,F-75015 PARIS,FRANCE

来源：

HUMAN GENETICS | 1995年 / 96卷 / 06期

关键词：

D O I：

10.1007/BF00210294

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The meiotic segregation of chromosomes 14 and 21 was analysed in 1116 spermatozoa from an oligo-asthenospermic carrier of a Robsertsonian translocation t(14q21q), and in 16 392 spermatozoa from a control donor, using two-colour fluorescence in situ hybridisation (FISH). Two YAC probes (cloned in yeast artificial chromosomes) specific for regions on the long arms of these chromosomes were co-hybridised. Of the spermatozoa, 12% were unbalanced, resulting from adjacent segregations. Chromosomes X, Y and 1 were also simultaneously detected in 1335 spermatozoa from the same carrier. Whereas gonosomal disomy rates were not significantly different from those of the control donors, disomy 1 were slightly but significantly increased to 0.7%. The diploidy rate was also slightly increased to approximately 1% in the translocation carrier.

引用

页码：655 / 660

页数：6

共 22 条

[1] SYSTEMATIC ANALYSIS OF 95 RECIPROCAL TRANSLOCATIONS OF AUTOSOMES [J].

AURIAS, A ;

PRIEUR, M ;

DUTRILLAUX, B ;

LEJEUNE, J .

HUMAN GENETICS, 1978, 45 (03) :259-282

[2] SEGREGATION OF CHROMOSOMES INTO THE SPERMATOZOA OF A MAN HETEROZYGOUS FOR A 14-21 ROBERTSONIAN TRANSLOCATION [J].

BALKAN, W ;

MARTIN, RH .

AMERICAN JOURNAL OF MEDICAL GENETICS, 1983, 16 (02) :169-172

[3]

CHEVRET E, 1995, IN PRESS CYTOGENET C

[4]

FORABOSCO A, 1973, ANN GENET-PARIS, V16, P57

[5]

Goldman A. S. H., 1993, European Journal of Human Genetics, V1, P325

[6]

GUICHAOUA MR, 1990, HUM GENET, V86, P162

[7]

GUTTENBACH M, 1994, HUM GENET, V93, P421

[8]

GUTTENBACH M, 1994, HUM GENET, V93, P7

[9] DETECTION OF CHROMOSOME 17-BEARING AND X-BEARING HUMAN SPERMATOZOA USING FLUORESCENCE INSITU HYBRIDIZATION [J].

HAN, TL ;

WEBB, GC ;

FLAHERTY, SP ;

CORRELL, A ;

MATTHEWS, CD ;

FORD, JH .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 1992, 33 (02) :189-194

[10]

HOLMES JM, 1993, HUM GENET, V91, P20

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