DIRECT SHOOT FORMATION IN SPONTANEOUSLY OCCURRING ROOT PSEUDONODULES OF ALFALFA (MEDICAGO-SATIVA L)

被引：9

作者：

SARUL, P

VLAHOVA, M

IVANOVA, A

ATANASSOV, A

机构：

[1] INST GENET ENGN, BU-2232 KOSTINBROD 2, BULGARIA

[2] INST FORAGE CROPS, ULAANBAATAR, MONGOLIA

[3] DE MONTFORT UNIV, NORMAN BORLAUG CTR PLANT SCI RES, BU-2232 KOSTINBROD 2, BULGARIA

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 1995年 / 31卷 / 01期

关键词：

ALFALFA; RHIZOBIUM; NODULATION; ORGANOGENESIS; ENDOGENOUS GROWTH REGULATORS;

D O I：

10.1007/BF02632221

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A simple and rapid procedure for direct organogenesis from root nodulelike structures of alfalfa (Medicago sativa L.) line SGg, spontaneously induced on growth regulator-free Gamborg (B-5) medium, was developed. Prolific adventitious shoot initiation was obtained using a combination of 1.0 mg/liter TIBA and 0.5 mg/liter 2iP. Transfer of shoots to a medium containing 0.5 mg/liter ABA and reduced concentration of TIBA (0.5 mg/liter) before rooting markedly stimulated shoot development. Regenerated shoots rooted easily and revealed the early appearance of nodulelike structures on basal medium (B-5) lacking growth regulators. Analysis of endogenous growth regulator levels of SGg roots maintained on growth regulators free media, showed that spontaneous shoot appearance was correlated with high cytokinin-to-auxin ratios.

引用

页码：21 / 25

页数：5

共 28 条

[1] REGULATABLE ENDOGENOUS PRODUCTION OF CYTOKININS UP TO TOXIC LEVELS IN TRANSGENIC PLANTS AND PLANT-TISSUES [J].