ANTISPERM ANTIBODIES INDUCE POLYSPERMY BY PROMOTING ADHERENCE OF HUMAN SPERM TO ZONA-FREE HAMSTER EGGS

被引：37

作者：

BRONSON, RA

FUSI, F

COOPER, GW

PHILLIPS, DM

机构：

[1] Division of Reproductive Endocrinology, Health Sciences Center, University of New York at Stony Brook, Stony Brook

[2] Laboratory of Human Reproduction, North Shore University Hospital, Manhasset, NY

[3] Population Council, Center for Biomedical Research, NY, NY

来源：

HUMAN REPRODUCTION | 1990年 / 5卷 / 06期

关键词：

Acrosome reaction; Antisperm antibodies; Fertilization; Oolemma; Spermatozoa;

D O I：

10.1093/oxfordjournals.humrep.a137169

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Certain antisperm antibodies (ASAs) in the sera of infertile men or women promote the penetration of zona-free hamster eggs by spermatozoa. We have shown previously that this enhancement of penetration occurs through mechanisms other than an alteration in the acrosomal structure of antibody-labelled spermatozoa. In the present study, small limited populations of antibody-labelled and antibody-free spermatozoa from fertile donors were observed serially by phase contrast, epifluorescence and scanning electron microscopy following their adherence to the oolemma of zona-free hamster eggs. The adherence of ASA-labelled spermatozoa to the zona-free hamster egg was markedly increased and occurred earlier when compared with antibody-free spermatozoa from the same ejaculate. The likelihood of entry of an antibody-labelled spermatozoon into the ooplasm, however, once adherent to the oolemma was not different to that of an antibody-free spermatozoa, and the uhrastructural steps of incorporation of antibody-labelled human spermatozoa were also similar to those observed during fertilization of hamster eggs by spermatozoa of several other species. These observations indicate that ASAs promote polyspermic fertilization of zona-free hamster eggs primarily through then-enhancement of sperm-oolemmal binding. © 1990 Oxford University Press.

引用

页码：690 / 696

页数：7

共 24 条

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